Journal: Biomedicines

Article Title: Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients

doi: 10.3390/biomedicines9070724

Figure Lengend Snippet: Flow cytometric analysis of Th cells. A demonstration of the expression of IL-4 and IFN-γ on phorbol myristate acetate and ionomycin-stimulated peripheral blood CD4 + Th cells from one non-TB subject ( a ) and one patient infected with TB ( b ). Peripheral blood mononuclear cells were stained with PerCP-conjugated anti-Leu-3a/CD4 antibody, FITC-conjugated anti-INF-γ antibody, and PE-conjugated anti-IL-4 antibody or the respective isotype IgG controls. The expression of IFN-γ and IL-4 on Th cells was identified by flow cytometry. Th, CD4 + T helper; TB, tuberculosis; PerCP, peridinin–chlorophyll protein; FITC, fluorescein isothiocyanate; PE, phycoerythrin; IL-4, interleukin-4; IFN-γ, interferon-γ.

Article Snippet: The cells were then incubated in the presence of monoclonal antibodies of anti-INF-γ fluorescein isothiocyanate (FITC; Becton Dickinson)/anti-IL-4 phycoerythrin (PE; Becton Dickinson) or the corresponding isotype controls in the dark for 1 h at 4 °C.

Techniques: Expressing, Infection, Staining, Flow Cytometry